Development history of TI Workbench

•  Event Ensemble Analysis was added.
  

•  Images embeded in MS Office files (.docx, .pptx, xlsx) can be directly opened.
  

•  Analysis of segments detected by watershed segmentation is now enabled. And numerous changes and bug fixes since 2018.
  

•  Digital filters were implemented in the electrophysiology data analysis.

• Labnote was added as an additional functionality to Layout.
• The Note window was renamed as "Log" window.
  

• Axon .abf file format export was added.
  

• "labnote" file format was added in addition to the "layout" format.
  

• "gaussian filter" was added to the spacial filters.
  

• Event detection function for time course plots of image data was partially working.  It was not available for ratio data.  Now it is fully working for ratio data as well as raw data plots.
  

• The off-line version is now running under 64 bit mode.  Carbon codes were replaced with Cocoa codes.  For on-line version Carbon to Cocoa transition depends on the compatibilities with device drivers.
• With the 64 bit version, large file handling has much less troublesome.
  

• A/D data is stored as compressed format.  A/D data saved with TIWB versions from this date can not be read with older versions.  Newer TIWB versions can read old data formats.
  

• The "Save As..." function for image data was moved to "Export..." in the "Export" menu.
  
• Olympus FV-1000 binary format files ("*.oif") are read in addition to the ".oib" file.

• libDC1394 Firewire camera library is supported, which enables many CCD camera types working with tiwb.
  

• A fatal bug was fixed which caused unstability in the slope detection function in electrophysiology. 

• Text can be put on the Layout window. 

• A/D conversion ratio of ITC-16 was changed from (1000 mV / 3000) to (1024 mV / 32768).  Accordingly converted voltage is 1.067 times larger than before.  This new conversion rate is correct.  A/D data taken with ITC-16 in older version of tiwb than this version will be displayed correctly (1.067 times larger) in this version.  ITC-18 has been used this (1024 mV / 32768) conversion rate in previous versions. 

• Drag'n Dropping the proxy icon onto Finder window or desktop creates a PDF file instead of a clipping file.

• A bug was fixed which prevent update data header in file in PPC Macs. This bug has been existed since tiwb was conformed to Intel architecture.

2/6/2010

• movie files can be read. .mov, .wmv, .mp4, .aif format files are read.

9/7/2009

• export to a PDF file is added to the OS-X version. PDF format is also added to the Clipboard when data window is "copied".

3/8/2009

• direct exporting data to Microsoft Excel is added to some of the export menus.

8/26/2008

• Real-time pixel intensity histogram is added to the Image Browser Window.
• Olympus FV-1000 binary format files ("*.oib") are read. This feature is available with a special version of TI Workbench and installation of the "libgsf" library package.

2/18/2008

• AppleScript support (starting from very small portion of the functions).

9/30/2007

• Prairie View Tiff format image stack is now readable.

8/14/2007

• In addition to the "Link and overlay" dialog, image overlay can also be done by drag and dropping the Proxy Icon of an image window onto a destination image window.
  

6/13/2007

• TIWB is now configured as universal binary and runs much faster on Intel Macs.
• Hamamatsu DCAM cameras and Photometrics PVCAM cameras can be used with Intel Macs.
  

5/20/2007

• PVCAM-handling routines were reviewed.  Some bugs, which had been arose from the transients of PVCAM from ver. 1.x to 2.6 or higher, were fixed.  Fast-continuous mode now uses the "circular buffer" mode of PVCAM when available, so that longer continuous acquisition is possible.  TIWB is now compatible with the QuanTEM line cameras.
  

11/15/2005

• There had been a serious bug in tiwb which adds unnecessary data (~1kbyte) to opened data files. Affected are from August to 11/4/05 versions. The latest version (11/15/05) fixed this. Even if a data file had been added such junk data fragments, this version ignore them, and the original data part are not modified at all. This bug had been introduced when tiwb was adapted to file size more than 1 Gbyte.

10/4/2005

• In bleaching correction, user can choose whether fit along each pixel or fit using sum of all pixels.

10/3/2005

• "Ratio-Intensity" display mode was added.
• Contents of the "Note" window" is saved automatically in the current preference file (periodically saved). The contents is not erased by closing the window. At the moment, the contents must be manually erased (select all (cmd+A), then press delete key).

8/10/2005

• MetaMorph format (STK) files can be read (not complete though).

6/3/2005

• Scroll wheel of mouse can be used in slide text edit.
• AppleScript-compatible. Many functions can be made AppleScript-compatible now. Request the author which functions should be AppleScript-compatible.

3/5/2005

• Long file name (> 32 chars) is allowed.

3/1/2005

• Scion Firewire cameras are supported for OS-X.

12/8/2004

• Auto focus module for Olympus IX microscope (IX81-ZDC) can be used for auto focusing.

11/26/2004

• QCam CCD devices by Quantitative Imaging Corp is supported for OS-X.
• Disk Scan Confocal system (DSU) by Olympus is supported.

9/21/2004

• The print function of electrophysiological data has been consolidated into the "Layout" window. Format of A/D data in Layout can be set through the "PICT format..." menu in the "Edit" menu. A/D data can also be Copied and pasted to other applications.

9/1/2004

• Electrophysiology: In OS-X versions, multiple wave display and averaging method was changed, which can be achieved through drag'n drop data items onto other items in the drawer. Refer to the manual.
• Electrophysiology: Experiment parameter set handling was renewed in the "Oscillo Panel" (OS-X only).
• Electrophysiology: The user defined constants (x, y, z) can now be set as arrays of numbers.
• Jaggy looking in rotated images has been dismissed by changing the rotation algorythm to use interpolation.

6/25/2004

• "Display linked AD sweep" menu was moved to "global plot parameters" dialog which can be selected through the option menu tag at the right-top corner of each time-course plot window.

5/24/2004

• Short cut key (cmd + right arrow key) to start/stop fast animation was added.

5/20/2004

• "Update Preference file" was added to the File menu.

4/5/2004

• Darg and Drop operation is available with the "Proxy Icon" in the window title to Drag and drop the PICT or TEXT information to other applications (Igor does not accept Drag and Drop, though).
• Most of the editable text information including the "Note" window can be drag'n dropped to other applications without copy and pasting.

1/29/2004

• "stack surface" 3D modeling function was added to the "3D display" function. Since this function is primitive, I welcome opinons of the users.
• A bug in image rotate was fixed, which had prevented proper image rotation by mouse drag.
• A bug was fixed in electrophysiology A/D recording in OS-X version which intermixed A/D channels.

12/18/2003

• QuickTime movie files made through the "save as" menu were not readable in Windows environment (Quicktime Player or Power Point), because quick time data was stored in the resource fork of movie files. From this version, quick time data is stored in data fork, which is readable in Windows environment. Movie files generated older versions of tiwb can be read in Windows environment by read and save as another file with "QuickTime Pro" player running on Macintosh OS.

12/4/2003

• Many parts of thesource code were revised. Accordingly, response and performance were greatly improved.

10/27/2003

• Different exposure time can be set to F2 and extra-1 wave length in discontinuous seq mode and taking a single frame pair mode.

9/9/2003

• image file format description was added, though not complehensive.

6/30/2003

• "exp set" menu was added to the "CCD online" dialog.
• The behavior of pressing the zooming button of image windows was improved.

5/12/2003

• "mask dimm pixels of dividing image after background subtration" option was added to avoid noisy background in ratio images.
• "Compress" option was added to save dialogs for image. Classic "Huffman" algorithm is used, which is very fast and modestly effective in compression.

4/2/2003

• Number of ROIs in an image is limitless (has been 20). However, location and shape of first 20 ROIs are recorded in data header or separage parameter files.
• rand() function is added to the stimulator description formula.
• Interval for free run or train run of Oscillo panel accepts formula. Together with the rand() function introduced this time, random interval stimulation is available.

3/3/2003

• Histogram is displayed for pixel density of images.
• Explanation for the bleaching correction section in the manual was updated.

2/24/2003

• Magenta was added to the color look up table (LUT). Use of Magenta instead of Red in presentations is encouraged for colorblind people.
• File contents can be previewed in file open dialog (OS-X only).
• "Memo Window" was renamed as "Note Window", and the behavior is improved. And text files can be opened and edited in separate windows to the "Note Window".

2/14/2003

• Scion LG-3 frame grabber works on OS-X.

2/12/2003

• Library for Instrutech ITC-16 and ITC-18 was replaced with a new version.  Previous version of the library was used since 1998.  The previous version of the library works without problems on Macintoshs with 604e CPU, but does not work properly with G4 Macintoshes.  For example, once TI Workbench was started and quited, ITC-18 freezes and rebooting of Macintosh after turning off the power was needed.  This problem was gone with the new library.
• "On-line filtering" function for image data is very useful especially for image ratioing with rather noise images.
  
• ORCA-ER camera (Hamamatsu) with Snapper frame grabber card (DataCell) can be used under OS-X.

11/5/2002

• "Easy Time Lapse" function is added.
• "full frame" can "take" multiple wavelength at once.

10/22/2002

• Animate of time lapse image stack loops the range matching to those in time course plots.

9/28/2002

• TIWB has added resouce fork to store several conditions (LUT, magnification, etc.) in foreign image files when "Save back parameters" option is turned on. This way causes data file crash if the data file is located in Windows machine and is accessed through Mac OS-X SMB file sharing machanism. So, additional information is stored in a separate file with extension of ".tiprm".

8/27/2002

• Location of temporal file for transfering data to/from Igor was changed from Igor application folder to system-defined temporal folder.

8/2/2002

• "show tomography" function was added to the local image option menu. This enables to show a cross section through time course or z-section.

6/7/2002

• In stimulator output, real wave form can be used for output pattern.

5/30/2002

• Storing and selecting "User setting" became convenient. Way of handling user settings has been like following. All the user settings and parameters was stored in "TI Workbench.pref" in "System folder"->"Preferences" folder by default. User settings was able to be saved to other files by "File"->"Save preferences..." menu. But, all the settings are saved to the default preference file when the program quits. Changes of parameters after saving a user-specific parameter file are stored in the default preference file instead of user-specific preference file. This version, once a user starts the program with a user-specific preference file, all the settings and parameters are stored back into the user-specific file used at start-up instead of the default preference file. So users can have different setting files for different experiments. The new rule is like this:
  • If tiwb is started with a preference file (by dropping a preference file onto tiwb icon or double-clicking a preference file icon), all the settings and parameters are stored back into the same preference file. The default preference file ("System folder"->"preferences"->"TI Workbench.pref") is not altered.
  • Tiwb refuses to be started with more than one preference files.
  • After starting tiwb, preference files can be opened by double clicking or droppiing their icons onto tiwb's icon to change (not all of) settings and parameters of the program. (This was also possible in previous versions.) But, settings and parameters are stored back not to parameter files opend during execution but to the preference file read at starting time.
• Another mode of time-lapse imaging function was added to the "full" image. This enables parallel time lapse of "seq" tand "full" with different intervals and camera conditions (binning, expose time, filter types, etc.).
• OS-X version is available for ITC-16 and ITC-18. But no camera drivers yet!
• The annoying "plot windown disapperance" problem should have gone.
• Many other bug fixes.

4/2/2002

• Some bottle neck parts are optimized for Velocity Engine (AltiVec), improving speed in displaying images and A/D sweeps.
• By virtue of the QuickTime importer, many kinds of image formats can be imported.

2/25/2002

• Copy and Paste functions for Image were consolidated. Copying ROI positions, ROI colors are all done with other properties by just selecting "Copy" menu item in the Edit menu (short cut: cmd + C). This Copy operation also copies a PICT of the image to clipboard which then used by other applications. Copied properties of image can be transfered to other images by selecting "Paste Special" (short cut: cmd + shift + P).

2/21/2002

• Most of the operations on images can be undo / redo (thorough undo or redo menu item in the Edit menu.
• "ROI is oval" menu item was moved from "glogal Image option" menu to "local image opton" menu. Whether ROI is oval or rectangle is stored in each image segment header.
• Bug fix: a serious bug was introduced in last October in focusing for Photometrics cameras.

2/11/2002

• Local Imge Option Menu was moved from image browser window and left bottom coner of each image window to the menu bar.
• Time mark in time coruse plot in the course of discontinous image sequence acquisition is available.

1/27/2002

• Help is available in many dialogs and in Help Menu with a web browser.

1/13/2002

• In analysis for image time course plots, time range can be modified by directly crick and drag the colored area in plot graph.

1/3/2002

• Bug fix: animaiton of ratio image was very slow.

10/17/2001

• Bug fix: speed factor for exorting QuickTime Movie did not work at all.

9/28/2001

• Size of font for ROI ID can be changed.
• Position tracking feature was added in Image windows.
• "train" mode was added to the CCD segments panel.
• "interleave exposure" option was added to the "link A/D with CCD" dialog.
• The meaning of words "interleave" and "interval" are defind for clarity.

8/28/2001

• Another mouse action was added to electrophysiology window: display range of time axis can be changed by dragging mouse in the graph box.

8/10/2001

• A bug was fixed by which "fitting using first and last values with exponential function" function in bleach correction functions for images were miscalculated.
• Methods to compensate bleaching have been different between "bleach correction with external file" and "bleach correction with first and last values of the same sweep". The methods used in previous tiwb versions were:
• 1) Methods for "fitting using first and last values with exponential function".

  F'(t) = F(t) * (fitfunc(t0) / fitfunc(t))

  F'(t) is compensated F value at time t. F(t) is a F value at given time t. t0 is the first frame lapse time. fitfunc(t) is a function with fitted parameters.

  2) Methods for "bleach correction with first and last values of the same sweep".

  F'(t) = F(t) + (fitfunc(t0) - fitfunc(t))

  In both cases, F(t) is F values after autofluorescence correction. Bleaching correction is caliculated for each pixel of image frame along the time stack, i.e., each pixel has different fitted functions.

  • Now, all bleach corrections are done by the former method.
    

8/6/2001

• Program behavior in adding ROI to images is changed when "use last ROI as background" option is on: Newly added ROIs are inserted before the last ROI, leaving the last "background" ROI the same. And the last ROI for background is always displayed by dotted black line.

8/3/2001

• Timing of filter change is changed to reduce unnecessary illumination. Filter is changed just before exposure start and then changed to dark position just before data retrieval to a host computer, if the camera hardware allows.
• Negative values can be set to the "delay" parameter for TIL-monochrometer configuration. When negative values are set to this parameter, light source change is delayed with this period unless light is turned off (dark position). This further decreases unnecessary illumination time before "actural" exposure. To enable this function, make sure that the wiring is done according to a section of the manual, "Both Photometrics PXL/Quantix and TILL Polychrome monochromator controlled with National Instruments PCI-1200".

7/18/2001

• overlay image can be rotated / expanded / shifted. Mix modes of images is selectable.

6/15/2001

• "tiled plots" function was added.
• "manual F2" is available.
• Exporting image data to igor got much faster.
• Many bugs were fixed.

5/29/2001

• "Tiled plots" feature is added for arranging time course plots of image data. This function can be accessed through the "local option menu" of image browser (short cut: cmd + T).

5/28/2001

• A bug was fixed which caused strange behavior in the "hardware gain" setting dialog called from the "Oscillo panel" dialog.
• A bug was fixed which caused incorrect automatic gain control for images. There is no problem in the accuracy of the data itself.

5/14/2001

• Size of the text buffer of the "memo" window was limited to 32 Kbytes. This limitation was removed.

5/9/2001

• A serious bug was fixed, which has caused unstability of the program.
• Exporting data to Igor got much faster than before. This was due to change exporting mechanism from PPC (program to program communication) mechanism of the Mac OS to AppleEvent mechanism.

4/18/2001

• All A/D channel data can be sent to Igor by one action.

4/5/2001

• A bug in electrical recording was fixed which caused strange behavior when long duraion (more than 2 sec) recording is halted by pressing stop button.

3/28/2001

• A single image can be used for autofluorescence correction.

3/15/2001

• Separate LUTs are remembered for ratio and raw images.

2/15/2001

• Window positions are properly remembered in multiple monitor environment.

1/29/2001

• A/D data can be live scrolled.
• Tiff 24 bit color files can be opened.

1/18/2001

• IMPORTANT. Since a part of the preference file format is changed, camera on-line parameters (INCLUDING filter exchanger settngs and camera gain, etc.) are reset when on-line program is replaced with older ones than this version into this or a newer version.

12/14/00

• Formats of file name and data id assigned by tiwb are changed. Now, date is expressed by two numerics and exprirmnt number is expressed by two numerics. IT IS VERY IMPORTANT that data files generated by this version or later ones MUST be opened by this version of later ones, otherwise old versions of tiwb will crash or change the data id stored in the data header. This version can open data files generated by older versions, of course.

11/30/00

• Bug fix. Improper behavior in handling "full frame" image (or "reference image"). The "full frame" image checkbox in the "Bleach and Autofluorescence" dialog and the "reference (full frame) image" option in the local image options should have been the same. This bug had been introduced since the version of 9/27/00.
• Linked "full frame" image information is stored in the original image file (resource fork), and "full image" is opened automatically when the original image is opened.
• Descriptions for "focus averaging" and "background subtraction in focusing" are added in the manual.

11/22/00

• A bug was fixed which caused improper behavior in "keep prev.." options for images. This bug had been introduced since the version of 9/27/00.

10/25/00

• A serious bug was fixed which caused crash when file name was chanegd in CCD conrol panel.

10/17/00

• Carbon version is available for off-line mode.
• A sort of "flat-fielding" operation can be performed. This is useful for enhancing contrast of DIC images.

9/27/00

• A bug was removed which has caused crashing system in acquisition time.
• Sequential image stack can be averaged before analysis through the average, bleach and autofluorescence dialog.
• Background subtraction function is deeply extended. Bleaching correction and autofluorescence correction can be done using external sweeps. Refer to average, bleach and autofluorescence dialog.
• Image contrast optimize button was addd to the rght top coner of image windows, which is equivalent to the "optimize" button in the image contrast selection dialog.
• In opening a file, particular packet(s) in a file can be directly chosen. Refer here.
• Linking image acquisition and electrophysiology is controlled in a separate control panel.
• Linked A/D sweep is now automaticlly opened when an image stack is opened. A/D file does not have to be opened prior to opening image stack.

6/10/00

• Image data can be sent to Igor as 2D wave (matrix) data as well as PICT image data. This enables further analysis of image data in Igor.
• 3D display function of Igor Pro (version 3.14 or later) is available in addition to TI Workbench's 3D display. These two functions can be switched in the "3D display" dialog.

6/5/00

• Scroll check box was added to the oscillo panel.
• history menu was added to the "full frame" box in the "ccd" control panel.
• A bug was fixed in layout window: copying to the clipboard with zoom factor less than 100% did not work properly. I also notice that there has been no description about that the "copy" and "cut" work differently from the Macintosh standard behavior, which has been added to the Layout Window section.

5/26/00

• Spot CCD camera (Diagnostics Instruments Inc.) is supported. At this time, just a single filter exposure is capable. Spot-RT is not supported.

5/13/00

• Troubles (mismatched contrast, LUT manipuration ) in displaying 8 bit images are solved.

5/10/00

• Sutter Lambda 10-2 filter/shutter controller is supported through serial connection.

5/1/00

• Scion LG-3 frame grabber card is now seriously supported. Refer to the newly added appendix for the different behavior for LG-3.

4/4/00

• "Misc-Option" was added to the menu bar.
• Tiwb warns when the Memo window is closed without saving the content. This warning can be turned on/off through option menu.
• Speech recognition manager can be used to order some functions by voice.

3/2/00

• For Photometrics PXL and Quantix users:
  • Blush up for Quantix cameras.
    • The exposure time in fast continuous mode of Quantix frame transfer cameras was found to be accurate. This means that an external clock control is not needed, which is necessary for PXL-SCSI connection cameras.
    • No crash with faster frame rate than machine limit.
    • Warning when frame rate is shorter than machine limit (fast cont mode).
    • "measure minimum" function is very accurate (only for fast cont mode).
  • It is now selectable whether to use external clock from PCI-1200 in the camera configuration dialog. If you have been using this function with PXL in older version than this, check "Trigger A/D start by PCI-1200 OUTB1 port on fast read mode" check box once. Quantix users should uncheck this option.
  • When Photometrics PXL/Quantix frame transfer cameras has a shutter in front of the CCD chip, exposure time for focusing was not controlled, which was corected. However, the shutter does open/close in each exposure in focusing. Due to this behavior, focusing does not make use of the benefit of frame-transfer. Fast read mode is not affected by this behavior. And PXL/Quantix frame transfer cameras without a shutter are free from this issue.
• TIFF files produced by Zeiss confocal microscope system can be read as a sequence. Files named with continuous numbers can be read as a series of images.
• Minor bugs are fixed.
  • TIWB crashed when height of an oval ROI was set to 1.
  • The last point of time course plot of an image segment was not displayed.
  • Contrast of "seq" image window was not automatically adjusted even when "auto gain" check box was checked in fast continuous mode.

2/4/00

• "Save As.." function was expanded for TIWB format files. When Save As TIWB format from TIWB format file, data segments to be saved can be chosen. This enables removing unnecessary data segments from a file. Thanks to Chris Leonard's comment.
• A newer library (PPK rev. F1) is used for compile of PVCam version. (PPK rev. F has been used.)

2/3/00

• Y2K bug was found and fixed. Weird year was displayed. Thanks to Chris Leonard's comment.
• A lot of mis-spelling was corrected in this manual.

12/27/99

• ARGUS image data format (*.img; Hamamatsu Photonics) can be read by tiwb.

11/14/99

• "Synchronization" section was added to the manual.
• Some annoying behaviors in some "slide text edit" controls are improved.

9/16/99

• In ratio display mode, "cut off dim pixel" mode was added to ignore pixels lower than the display threshold.
• Any frames (single frame or averaged frames) can be used as Fbase.
• Image frame stack can be processed in the "filter" function.

6/10/99

• "Save as TIFF" function was improved: multiple stack can be saved in several bit depth.
• "Save as..." dialog had bugs with Navigation Service of Mac OS, which was fixed.

5/5/99

• A ROI in an image can be activated (blinking) just touching inside the ROI.
• Font size of memo text can be set when an image information is exported to Igor.
• delta F / Fbase display is available. % display is also available.

4/26/99

• User can change subregion size, binning factor and cut-off limit during focusing without terminating focusing.
• Cut off limit can be set in auto gain mode in focusing.
• A/D oscilloscope does not stop when image focusing is updating when A/D is running in auto trigger mode. This feature is useful for making patch clamp with monitoring with a camera controlled by this software.
• Line thickness can be chosen when plots are sent to Igor.

3/24/99

• Princeton CCD cameras are now seriously supported.
• Statistical analysis items are added to time course plot analysis for image.
• hardware information of a camera (gain, A/D speed, etc) is displayed in the info box of ImageBrowser. This function is available for data taken before this time.

2/10/99

• Sensicam CCD camera (PCO) is supported.

1/9/99

• Data format for Ross Lab is readable.

12/28/98

• ROIs can be edited by keyboard (edit ROI... menu item in the local image option menu).
• Fitting to exponential curves is available in image time course plot analysis.
• A subtle bug, by which "File I/O error" message was unnecessarily shown, was fixed.

8/20/98

• ROI ID can be displayed in the image window. Use "show ROI ID" menu item in the image option menu (menu bar).
• Length is an image can be measured by clicking mouse with shift key down. The result is displayed in the Memo Window.
• More Filter Functions are added to the image filter.
• Analysis functions are added to the image plot, which can be accessed by clicking inside the plot windows.
• Handling of display range of image time course plots is improved. "full range" button was added and slide text edit is supported to the disp range text edits.
• "search a boundary line" item was added to the "Analyze Image" dialog.
• A bug was fixed: in some situation, the program hung up in exporting sequential image data to Igor.

8/2/98

• Thanks to Lucas's comment, a serious bug was fixed: The values in the plot of detected slope (negative or positive) in the electrophysiology part was wrong. The values should have been the largest voltage (or current) difference divided by the "slope detection width" time. But they have been the largest voltage (or current) difference values. They were not divided by the time. So the previous "wrong" plot is linearly correlated to the "correct" plot. The slope value displayed in the "Peak Window" was correct.

7/27/98

• "peak" mode was added to the peak detection function of the electrophysiology functions. This mode takes largest peak irrespective of its sign (negative or positive).

7/21/98

• Continuous mode is now available for frame transfer type PXL series cameras controlled with National Instrument's PCI-1200 card. Since this mode uses Macintosh's timer interruption, mis-acquisition of a frame could possibly occur especially in short exposure time conditions. Please feed back to the author about the successful rate of this mode.

7/15/98

• A serious bug was fixed: Pushing "take" button for full frame acquisition does not return control to user in some cases. This bug seems to have been introduced during recent 2-3 months.
• A bug was fixed: When "Save as..." or "Copy" was selected in Layout window, not all images were saved / copied to Clipboard.
• A minor bug was fixed: display time range of A/D data was not correctly saved (in recent versions (several months?)).

7/1/98

• "Trigger A/D start by PCI-1200 OUTB1 port on fast read mode" function was added for Photometrics PXL series cameras controlled with PCI-1200.
• Mask operation in image was improved. User can now add/remove masked area manually. And pixel values in masked area can now be neglected by setting "ignore masked pixels" option in the background subtraction dialog.
• In "analyze..." dialog in the local image option menu, "regression line from pixel positions of which values in display range" item was added. It is a special purpose function which may not be useful.

6/16/98

• TIFF and PICT formats are added to the "Save As..." format for image.

6/5/98

• Sequential image acquisition is now controlled by Thread Manager of the Mac OS, which enables more quick response in fast rate acquisition in discontinuous mode.

5/1/98

• "fit plane" function was added (please wait for the explanation).
• "line compress" function was added (please wait for the explanation).

3/28/98

• "display baseline" switch was added to the Event Detect Dialog.

3/20/98

• The change on 3/2/98 was not true; on-line parameters has not been retrieved. This version enables that function. Preference files produced by previous version of this software hold on-line parameters (retrieving of their information was failed).
• With a frame transfer type ccd camera, exposure time in focusing mode is longer if too short exposure time is set (as indicated "actual duration" in red ). When such focused images were saved, "duration" in the file header held not the actual duration but "set" exposure times. This issue is corrected; data header of full frame images hold actual duration.
• Clicking Zoom box of the Memo window resulted in very huge size of window. This bug was fixed.
• More dialogs support baloon help.

3/17/98

• Sending pulse count of event detection to Igor is available (it has not been available).

3/14/98

• Manual was updated and the format was changed to HTML.
• A bug was fixed in 3/11 version: on-line event detection switches in oscillo panel could not be used.
• Stimulator parameters are also loaded when "load pref file" is done (it was not loaded so far).

3/11/98

• There was a bug in versions of Feb - March 2, '98: "save back header" option did not work in some situations. This was fixed.
• Event detection is available in on-line A/D acquisition.
• Event Count function was added to the Event detect function of A/D data.

3/2/98

• When a preference file is opened at start up time of TIWB (i.e., a preference file is dropped onto TIWB icon or a preference file is double-clicked when TIWB is not yet opened), on-line parameters are also loaded. (Thus far, on-line parameters can not be loaded by opening preference file.) On-line parameters is not still loaded when a preference file is opened after TIWB is already opened.

2/10/98

• "ignore pixels of which value below display range" switch was added to the background subtraction condition dialog in the local image option menu. With this switch on, pixels of which value under the lower display range are ignored when mean of ROIs are calculated. Background subtraction is done independent of this option.

2/7/98

• Princeton CCD cameras can be used.
• A selected ROI can be duplicated through "duplicate active ROI" item in the local image option menu (short cut: cmd+r).
• "analyze" item in the local image option was added, which enables to calculate stdev or rms of pixel values, etc.

1/23/98

• Scale can be displayed on image windows. Use "Set Scale..." item in the local image option menu.
• Way of interruption was changed for discontinuous mode of image acquisition, which enabled smoother user interaction during image acquisition.

1/8/98

• A movable dialog pops up on fast animation.
• Keep previous Mask option did not work, which bug was fixed.
• Many other subtle improvements and bug fixes.

12/25/97

• preferences can be saved/loaded. Use "save pref as..." item in the file menu to save a personal preferences for image and a/d settings. Saved preferences can be retrieved by opening the pref file.
• ROI handling by mouse was improved (more smooth).
• Comment of linked A/D data can be sent to Igor when time course plots of an image is sent.

12/12/97

• "to comment" button was added to the stimulator, which option adds stimulator command strings to the ad data comment.
• A bug in the stimulator command interpreter: commands after a loop expression were ignored (e.g. in case of "[0,1000;1,10],2;3,1000", the expression "3,1000" was ignored). This bug was fixed.
• Baloon help is available in "oscillo panel" and "stimulator".

12/1/97

• 3D display is available when QuickDraw 3D is installed (Power Macintosh only). "3D options" and "3D display" items are added to the local option menu of image window. Practical pixel size seems to be less than 100 x 100. 3D construction of the larger pixel image would require much time and memory. To reduce the pixel size, increase "smooth factor" for x and y coordination in the "3D options" dialog, or reduce the area. In the current version, if there is an active ROI (blinking ROI), 3D display is restricted to the active ROI (this feature can be changed in future). 3D displayed image can be rotated by dragging the mouse in the 3D window. The distance to the 3D object can be changed by dragging the mouse with a shift key pushed.
• Display parameters set by the user can be saved back to the file in case of TIFF format files.
• Image loading can be interrupted by pushing cmd+'.' keys when reading a huge file. Hold down the keys until loading quits.
• Default ROI color set can be changed by the user, in addition to the "ROI color copy/paste". The former is remembered after this application finishes/starts. This function can be accessed in the "ROI color..." item in the local option menu of image window.

11/12/97

• User can choose whether smoothen wave before measure slope of A/D data or not (previously always smothened).
• spacer between items can be chosen from comma, space and tab in memo out of image data.
• ROI shape can be copy/paste among image data.

10/16/97

• Bug fix: comment box of A/D dialog has malfunctioned for about a month.
• Bug fix: memo format dialog did not work properly.

10/9/97

• A bug was fixed: image size was not correct when scrolled, so that ROI position was confusing.

10/2/97

• Clicking close box with option key pushed closes all windows.

10/1/97

• In discontinuous mode of PVCAM cameras, way of periodic interruption was changed to avoid crashing. However, periodicity became bad, i.e. if Mac is busy (background application or user manipulates control panels in TIWB), exposure interval is delayed very easily. Anyway, the time course plot is plotted correctly, since exact exposure time is recorded in milisec precision.
• minor improvements.

9/15/97

• A bug was fixed: time unit was incorrect (1000 times larger value) in exported time course plot of image, which bug was introduced since 7/30/97 version.
• image window and "Layout" window are scrollable. The behavior of the grow box (right bottom corner of windows) of image windows was also changed; it does not change zooming factor but merely changes the window size.
• F1 and F2 mode was added to the image display option in addition to the "raw", "ratio", "F1+F2" and "mask" modes.
• "play button" was added to the image window, by which "fast animation" is started.
• "F - F0" (not F/F0, F1/F2, etc.) option in ratio parameter was added.
• A minor bug was fixed: "default param" in Calcium conc. dialog could not be remembered.
• File location information of the Igor program is now stored in the preference file "TI Workbench.pref" file in the "preference" folder in the system folder. So that "inoueIgorLocation" file in the "preference" folder is no longer necessary. Igor location is asked once again when this version is used.
• Igor communication part was improved. Frequency of the error message suggesting restarting Macintosh is reduced. Igor can be quitted during execution of TI Workbench.

9/4/97

• Discontinuous mode of sequential acquisition of PXL series camera is more stable, especially in "busy" conditions such as very short interval.
• ROI shapes can be set different among frames in a segment, which enables tracking a moving samples. Refer to "Segment plot" in the "ROI manipulation and Time course plot windows" section of the manual.
• "ROI shape is fixed" option was added to the "image-option" menu in the menu bar, which disables ROI shape change, but ROI movement and assignment of new ROIs can be done.
• Changing frame in a segment can be done by left and right arrow keys.
• "dark between" check box in the CCD control panel has not functioned, which now properly works.

8/10/97

• linked A/D sweep can be displayed in time course plots of an image data. "display linked AD data" option was added to the local image option menu.

8/7/97

• A bug was removed: too much focusing had caused crashing.
• Focusing speed was improved for frame transfer type cameras (especially binning larger than 2 x 2), but, instead program does not respond to user's action during focusing.

7/30/97

• Image option menu in the menu bar was separated into two menus. "Global" items which are used for all images remains in the "image-option" menu in the menu bar and "local" items which affect just for the active image were moved to "option" menu in the "image browser" dialog.
• Time unit can be chosen when A/D data or time course data of image is sent to Igor.

7/26/97

• Behavior in recording long duration A/D is improved. (1) It displays continuous (not dotted) sweep. (2) It does not display "junk" sweep when long recording is terminated before preset duration. (3) Display range (time and voltage) can be changed in recording. And subtle improvements.

6/28/97

• A serious bug was fixed: when quitting this application, crash or abnormal termination has been seen, which was fixed.
• Flickering seen in drawing A/D graph with peak detection range was removed.
• Intrinsic video digitizer function (AV-Mac) is again available.
• Fuji Deltaron fast camera is available.

6/14/97

• Short cut cmd+K is assigned to the "set mask" function in "image-option" menu.

5/16/97

• In alternate filter mode and ratio display mode, image was not displayed properly, which was fixed.

5/14/97

• "Event Detection" function is added to the A/D browser, which enables spontaneous EPSP counting, single channel event detection, etc, which can be called from "Event Detection" menu in the "ad-option" menu. However, there is still no description in the manual.

5/3/97

• An A/D, D/A card, Instrutech ITC-18, is available.
• Cancel button was added to the "Discard or save" dialog appearing in CCD online mode.

4/21/97

• ROI color change function was moved from "double clicking a Roi" to "image-options" menu.
• When time course plots are exported to Igor, each plot has separate lapse time list in Igor (they shared the lapse time list thus far). By this change, ratio plot does not have unnecessary points when F2 first and/or F2 last mode is selected.

4/19/97

• "optm" buttons in the A/D dialog sometimes caused hung up, which was fixed.

4/14/97

• So far, "TI Workbench" application file has been easily collapsed, because layout window used the resource folk of the application file as a buffer. This version uses a separate file as a layout window buffer.
• Modification of "auto track" function on 4/11 was not complete. Completed.
• More number of errors are reported by error messages instead of a simple beep.
• Type of items of the memo out function of Image can be chosen by the user.
• Memo text can be sent to Igor when an Image is sent to it.
• File plot can be raised to a Layout in Igor when exported.

4/11/97

• The "auto track" function in the oscillo panel was improved. It does not miss sweep.

4/5/97

• Way of automatic naming of full image was changed. When "save" button in the full image panel is pushed, the same name as the current seq data id (with ".dts" at the end) is assigned to the file name of the full image. User can of course change it.
• When time course plots of image frames are sent to Igor, linked A/D data can be exported with it.
• Ratio image display mode is available in the online "seq" image.

3/28/97

• A bug in ratio image was fixed. Ratio images of previous versions were incorrect when (1) "last ROI is background value" option is selected and not alternate filter mode and (2) single wave length mode and "fixed background value" or "last ROI is background value" mode was selected. Time course plot has been correct.

3/21/97

• An image segment keeps a data ID of a corresponding A/D data (termed "linking"). This link is made automatically when "link oscillo" and "auto save linked A/D" check boxes in the ccd dialog are on. Or, link is manually set/reset through "overlay and link..." item in the "image-option" menu. If an image segment has a link to an A/D data and the A/D data file is opened, corresponding A/D sweep is called automatically.

3/19/97

• The option "ratio plot inverted ordinate" added on 3/14/97 was removed. Instead, F1/F2 or F2/F1 can be chosen in "ratio and [Ca] parameters..." item in the "image-option" menu.

3/17/97

• Parameters for Ca conc. calculation can be (1) remembered as a default set and (2) kept among segments. To set/retrieve a default set of Ca parameters, choose "Ca" menu in the "image-option" menu. To on/off the keep option among segments, select "keep Ca param" option in the "image-option" menu.

3/14/97

• A bug was fixed: the ratio plot of the image time course was not correct when F2 first is checked. The plot was merely the plot in which every F1 value was divided by the first F1 value.
• Ratio plot can be displayed upside down. Select "ratio plot inverted ordinate" item in the "image-option" menu.
• A/D peak dialog is updated when oscillo window reads a new data, which enables resistance monitor when sealing a patch pipette or watching Rm or Rs.
• file plotting can be aborted by cmd + '.' keys.

3/9/97

• Items in the Layout Window can be resized by dragging the corners with mouse.
• "Record" button was added to the stimulator dialog, which is equivalent to the "Record" button in the Oscillo Panel.

3/5/97

• find button was added to the A/D dialog, which enables finding appropriate offset without changing gain.
• Bugs were fixed.
  .When full image was linked with the auto read of seq image, the interval of seq images (segments interval) were longer than the set value.
  . In Oscillo panel, when stimulation set was changed, data id was changed to illegal strings.

2/21/97

• A bug in "A/D print" function was fixed: 2nd channel data could not be printed properly.

2/20/97

• Scion LG-3 frame grabber is available for image acquire.
• Multiple subarray in fast continuous mode in PXL was not available because of a bug. It has been fixed.
• way of selecting object in Layout window was improved. (drag multiple objects, select/deselect object, etc.)
• Roi frames are updated in both full and seq image window in synchrony.

2/14/97

• Roi color can be changed by double clicking a Roi.
• A/D acquisition is more stabler (interrupt handling has been improved).

2/5/97

• Timing of A/D and image acquisition. plan 1. was to enable user defined "wait time" before A/D start. plan 2. was that A/D starts when F1 image acquisition starts. The latter (plan 2) was chosen because there is no way to detect if the "user defined wait time" is too short or not.
• Brightest 5 pixels are neglected from automatic guessing gain in focusing mode and monitoring time of discontinuous mode. This might reduce "jumping" gain.

2/3/97

• Overlay function was added to the Image functions, though it is not complete, at least you can overlay multiple images onto one image to make multiple staining sample to show multiple colors.

1/31/97

• F1 and F2 images can be displayed with separate LUT conditions.
• F1 and F2 images can be overlaid by "F1 + F2" display mode.
• Images are sent to IP-Lab through TIFF format temporally files instead of IP-Lab format files, which decreases hanging up of the computer, but LUT information can not be transmitted to IP-Lab.
• Pixel values of F2 images can be correctly displayed when mouse cursor is placed over the F2 image region in alternate filter mode.
• Roi can be selected/modified on the F2 image region in alternate filter mode.
• Red and green Cluts are added.

1/26/97

• Comment can be added/edited in the ImageBrowser dialog.
• Baloon Help is available in some menus and dialogs (currently, A/D browser, Image browser, A/D-option menu and image-option menu.
• Scroll bars are added to the comment box in the A/D browser and info. box in the Image browser. Comment can be directly modified in the comment box in the A/D browser.
• memo button in the A/D browser was deleted.

  
  1/21/97

• A/D data larger than 20 MBytes could not be read, which was fixed.

12/18/96

• The English of the manual was intensively blushed-up by Dr. Lucas Pozzo-Miller.
• QuickTime Movie output was improved. Image frame shape was not correct, which was fixed. Video play speed can be chosen.

12/12/96

• Layout Window is available. Images can be ported to it then can be printed / copied / saved.
• linking full image acquisition with automatic segment aquisition ("segments" acquisition).
• polarity of D/A output for TIL was again reversed. And the input voltage value must be the "final" voltage which is put into TIL (amplified voltage).
• viscosity correction factor is introduced to the Ca conc. calculation.

12/4/96

• TIFF file of Olympus Fluoview (confocal microscope) can be read.

12/1/96

• Memory allocation method has been improved, which results in reducing "memory shortage" error message.

11/29/96

• Slope detection is available in peak detection function of electrical recording.

11/26/96

• Ca conc calculation is available by Rmax/Rmin method. Use "[Ca]" menu item of the image-option menu.
• minor bugs are fixed:
  1. "fixed background value" text box works properly.
  2. "extra filter 3" check box can be properly set/reset.

10/29/96

• Empty disk space is correctly displayed when current disk is changed.

10/27/96

• Stop and Pause button is available during discontinuous recording of images of PXL series cameras and during all sequential recording of Series 200 cameras.
• "slow continuous mode" of PXL series camera is now not available because of its unreliability in exposure duration.
• mouse selection can be used either for multi ROI or sub register operations on the full image window, which can be chosen by the ratio buttons in the "ccd online" panel (and which is further reversed by pushing shift key).

10/24/96

• minor bug fix. When disk space is short, header has been written without writing data, which have made an "incomplete" file. "Header only file" is no longer made.
• disk space indicator in the ccd online panel sometimes displayed another disk's free space. This point was fixed.

10/23/96

• Disk full error is indicated (it has not been reported when saving).

10/20/96

• Mask function was added to the image functions. Images can be masked. For detail, refer to the manual.
• A bug was fixed: when Lut range is changed by sliding the LUT tag, this program hangs up when min and max tags cross.

10/19/96

• A fatal bug was fixed: when one image is opened, this program hung up when the image is closed!

10/18/96

• Ratio images have not been displayed correctly (deformed), which bug was fixed.
• Slide Text Edit input method can be used for the user variables in the stimulator.

10/1/96

• Way of background subtraction can be selected. (see "background subtraction" in the image-option Menu section of the manual). Thus far, this was fixed to the condition when image was taken.
• Time course plotting of raw image now displays background values by dotted line.
• Bugs were fixed.
  1. "use back image as 1st frame" is dimmed and not accessible in some situations.
  2. When ratio display is selected for an image of alternate filter mode, image window size was not changed to a single image size.
  3. When ratio display is selected, pixel value indicated by mouse movement was still that of raw data.

9/26/96

• When imaging and electrophysiological recording are combined, time stamp (experiment time) was not matched between the two recordings, which was fixed.

9/21/96

• Alternate filter mode is now available in fast continuous mode, when PXL and TIL Polychrometer is driven by National Instrument PCI-1200 card.

9/13/96

• "keep previous Rois" option was added to the "image-option" menu.

9/10/96

• Copy command copies image instead of LUT color belt when image browser dialog is active. To copy LUT color belt to the Clipboard, select "copy LUT to clipboard" in the "image-option" menu.

9/9/96

• Stimulator description syntax was expanded: refer to the corresponding part of the manual.
• TIL polychrometer can be controlled by DA port from ITC-16 for PCI Mac, although simultaneous electrical recording is not available.
• A bug in background subtraction was fixed.

8/30/96

• ITC-16 for PCI Macintosh can (seem to) be used with this program. Apparent problem: frequency more than 1 msec can not be used.

8/14/96

• "File Plot" function was added for time course plotting.
• Animation speed was improved.

8/6/96

• A fatal bug which caused crash after stopping sequential image acquisition was fixed.
• Live video (through video in port of AV Macs) image can be saved.

8/1/96

• auto track switch was added to the oscillo panel.
• "keep among experiment sets" switch was added to the hardware gain dialog of the oscillo panel.

7/24/96

• Igor Pro 3.01 was confirmed to work with this program.
• Stimulator parameters can be registered/retrieved.

7/23/96

• Linking of CCD recording and electrophysiological stimulation/recording is available.

7/22/96

• Oval ROI in the image windows is available.
• Current frame is indicated by a vertical line in time course plot windows. And the vertical line can be dragged to change current frame.

7/21/96

• ITC-16 for PCI card is supported.
• "slide text edit" was added to some text edit boxes in dialogs.

7/17/96

• "use shutter in CCD" button in the CCD configuration dialog for PXL had not worked, which was fixed.
• In focusing with frame-transfer type PXL camera with a shutter in the front, shutter did not open, which was fixed.

7/16/96

• "full" switch was added to the "full" panel in the CCD control dialog. With this switch off, sub array(s) is (are) taken as "full image" . This option was added for speed up focusing.

7/10/96

• Alternate filter image selection was added to the PICT option dialog.

7/8/96

• a vital bug was found and fixed, which has been causing memory shortage often.
• PICT options is added into the image-option menu.
• "use back image as first dark image", which was buggy, was corrected.
• "save" buttons in CCD dialog are dimmed after saving.
  * Final definitions (exact functions) of "keep previous display range" and "keep previous position" in the image-option menu have not been decided.

before 7/8/96

• Files made by this software can be opened by double clicking the data files or drag-and-dropping to the application icon.
• many changes.